Production and Characterization of an Antiserum against Recombinant Porcine Follicle Stimulating Hormone.

Production and Characterization of an Antiserum against Recombinant Porcine Follicle Stimulating Hormone

This study aimed to develop an antiserum specific to follicle stimulating hormone (FSH), for a wide range of species, free from cross-reactivity against luteinizing hormone. An antiserum against recombinant porcine FSH (rec-pFSH) expressed in insect cells was raised in a rabbit and its specificity was characterized by time-resolved fluoroimmunoassay (TR-FIA) and immunohistochemistry. The titer ...

Physicochemical Characterization of Pituitary Follicle-stimulating Hormone

The physiochemical characteristics of the follicle-stimulating hormone (FSH) from whole sheep pituitary glands have been studied. The hormone behaves as a single protein in electrophoresis, diffusion, and ultracentrifugation. It has an isoelectric point at pH 4.5 and a molecular weight of 67,000 and contains 1.23 per cent hexose and 1.51 per cent hexosamine. The amino acid composition has also ...

The Correlations of Anti-Mullerian Hormone, Follicle-Stimulating Hormone and Antral Follicle Count in Different Age Groups of Infertile Women

Isolation of the luteinizing hormone and follicle-stimulating hormone-releasing hormone from porcine hypothalami.

A new procedure is described for the isolation on a preparative scale of a hypothalamic decapeptide which stimulates the release of both luteiaizing hormone (LH) and folliclestimulating hormone (FSH) from the pituitary. The isolation was achieved mainly by countercurrent distribution. The yield from 240,000 porcine hypothalami was 11.4 mg. Thin layer chromatography and electrophoresis showed th...

Meta-analysis on recombinant versus urinary follicle stimulating hormone.

A recent meta-analysis (Daya and Gunby, 1999) on recombin-ant versus urinary follicle stimulating hormone (rFSH versus uFSH) deserves methodological comment. The a priori definition of eligibility criteria for studies to be included in a meta-analysis are central and must be systematic. It was up to the authors to decide whether their search would be extended to include all unpublished studies,...